Composite
INT5TLU1
Part:BBa_K3254026:Design
Designed by: Yeqing Zong, DENG,Gabriel Cheuk Yan Group: iGEM19_GENAS_China (2019-10-18)
Translational Unit for Int5 integrase
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 131
Illegal PstI site found at 1525 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 131
Illegal PstI site found at 1525 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 126
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 131
Illegal PstI site found at 1525 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 131
Illegal PstI site found at 1525
Illegal AgeI site found at 118
Illegal AgeI site found at 502
Illegal AgeI site found at 877 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The RiboJ part was added before the RBS-coding sequence, so that the modularity would be improved.
Source
Assembled by this group.